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Dr. Antonella Bugatti - Macromolecular Interaction Analysis Unit

Dr. Antonella Bugatti

Personal data

Born on November 12th, 1974 in Brescia, Italy. Italian citizenship.

Education

She gained her degree at the School of Medicine, University of Brescia, Italy, in 1997 (110/110 cum laude).
She is now a fellow researcher at the Section of Experimental Oncology and Immunology, School of Medicine, University of Brescia.

Her researches has focused on

1994-97: regulation of the gene expression of HSP70 protein family in different rat tissues in normal and pathophysiological conditions.
1997-00: biochemical studies for the production of biotechnological heparins.
2001-02: Tat-antagonist activity of polysulfonated/polysulfated compounds.
2002-04: pentraxin 3/FGF2 interaction.
2004-present: protein-protein interaction by surface plasmon resonance.
She is author of 17 scientific publications in peer-reviewed journals.

Technical skills and competences

Biochemistry: Utilization of BIAcore X and X100 strumentation for the study of molecular interactions by surface plasmon resonance and of dedicated software for data analysis. Protein extraction from tissues and cultured cells, chromatogragraphic methods (affinity, ion exchange, gel filtration, FPLC systems). Production and purification of recombinant proteins, Silver Stain, Western Blot, ELISA assays, DNA and RNA extraction, PCR.

Cell Biology: Colture of immortalized cell lines, isolation of endothelial cells from umbilical cord, cell proliferation, adhesion and migration assays, in vitro angiogenesis (matrigel, fibrin gel, collagen gel assays), transfection, immunofluorescence.

Publications not dealing with SPR

(see also Publications containing SPR analysis)

M. Belleri, D. Ribatti, M. Savio, LA. Stivala, L. Forti, E. Tanghetti , P. Alessi, D. Coltrini, A. Bugatti, S. Mitola, S. Nicoli, V. Vannini, M. Presta . “alphavbeta3 Integrin-dependentantiangiogenic activityof resveratrolstereoisomersMol Cancer Ther. 2008 7:3761-70. (I.F. 5.226)

M. Donalisio, M. Rusnati, V. Cagno , A. Civra, A. Bugatti, A. Giuliani, G. Pirri, M. Volante, M. Papotti, S. Landolfo, D. Lembo.

“Inhibition of human respiratorysyncytialvirus infectivitybya dendrimericheparan sulfate-bindingpeptide”  Antimicrob . Agents Chemother. 2012, 56:5278-88. (I.F. 4.565)§